Gulyamova1 1department of biochemistry and biotechnology of physiologically active compounds, institute of microbiology of the academy of sciences ru, uzbekistan. Antimicrobial activity in cultures of endophytic fungi isolated from. Pdf isolation and identification of endophytic fungi. Molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia. Antimicrobial activity of endophytic fungi the antimicrobial activity of 21 endophytic fungi was tested and shown in table 4. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional.
Occurrence, classification, function and natural products. The seven endophytic fungi were subjected for antimicrobial activity against pathogenic bacteria viz. Mycelial extracts mc and fermentation broth fb were tested for antimicrobial activity using peptide deformylase pdf inhibition fluorescence. Isolation and antibacterial activity of endophytic fungi. Biodiversity of endophytic fungi in different leaf ages of. The etoac extracts of all endophytic fungi and isolated compounds were screened for antimicrobial activity against s. Antimicrobial and enzyme activity of endophytic fungi isolated from tulsi. Study on angelica sinensis endophytic fungi and its. Antimicrobial activity screening of endophytic fungi extracts. Antitumor and antimicrobial activities of endophytic fungi. Jan 02, 2016 endophytic fungi were tested for antifungal activity using a dual culture method.
The current study determined the antimicrobial properties and identify the chemical compounds of secondary metabolites produced by endophytic fungi isolated from sceletium tortuosum l. Detection of secondary metabolites content of each endophytic fungi species. The fresh mycelia of endophytic fungi were grown on pda plates at 282. In this study, we evaluated the antimicrobial activity of endophytic fungi isolated from h. Each endophytic fungi isolates were inoculated in potato dextrose broth pdb.
Endophytic fungi isolation from the meliaceae plant xylocarpus granatum was investigated and 20 potential pure strains were isolated. The agar plate diffusion assay was used to evaluate the antimicrobial activity rios et al. Natural products from entophytic microbes have been observed to inhibit or kill wide variety of harmful disease. Nov 15, 2012 the antimicrobial activity of all 53 endophytic fungi was evaluated against six human pathogens xing et al.
Endophytic microorganisms are fungi or bacteria which live inside plant tissues or organs, without causing them any harmful symptoms. Endophytic fungi that showed antimicrobial activity were identified based on analysis of the dna sequences of the its15. Biodiversity and antimicrobial activity of endophytic fungi. In total, 382 filamentous fungi and yeast isolates were obtained and cultured to. About 17 8095% endophytic fungi showed activity against one or more bacteria, and 14 6667% endophytic fungi exhibited activity against one or more fungal pathogens. The bioactive abilities of endophytic fungi were not identical, even though they were isolated from the same host species, and belonged to the same genus. We conducted a study to bioprospect for saponinproducing endophytic fungi from panax notoginseng and evaluate the antimicrobial activity of saponins.
Pn17, were isolated from traditional chinese medicinal herb p. This could be because the endophytic fungi and plant pathogenic fungi naturally exist in the same habitat. This study shows that the endophytic fungi isolated. Biodiversity and antimicrobial activity of endophytic. Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant trichilia elegans a. Antimicrobial assay thirty nine isolates of endophytic fungi were randomly chosen to estimate their ability to produce antimicrobial compounds antibacterial or antifungal activity. Antibacterial and antioxidant potential of endophytic fungi. Antimicrobial activities of endophytic fungi isolated from ophiopogon. Endophytic fungi exhibiting broad spectrum of antimicrobial activity were subjected for the production of secondary metabolites. The present study helped to justify the traditional use of vitex negundo l. Pdf isolation and identification of endophytic fungi from.
Antimicrobial activity of crude extracts of endophytic fungi. Antimicrobial activity of endophytic fungal islate in argemone maxicana. Hence, in this paper we describe the isolation and identification of the endophytic fungi of the aquatic plant n. Method endophytic fungi were isolated from the stem, leaves and root of angelica sinensis by the surface sterilization method and scoring method. These microorganisms can be useful for prospectionof bioactive. C for 36 days and were inoculated into ml flasks containing 200gms of unpolished rice, soaked with 200 ml distilled water autoclaved. Pdf antibacterial activity of endophytic fungi isolated from conifer. Endophytic fungi have the ability to coexist with their host plants without causing any harm and are beneficial to both the plant and the fungi. Antimicrobial activity and phytochemical analysis of crude.
The present study is aimed at the isolation and identification of endophytic fungi from p. Evaluation of antimicrobial activity of endophytic fungi from. Pdf antimicrobial activity of endophytic fungi isolated from. The rafflesia flower is the largest single flower in the world, a parasitic flowering plant found only in borneo, indonesia, malaysia and philippines. Antimicrobial activity of kojic acid from endophytic fungus colletotrichum gloeosporioides isolated from sonneratia apetala, a mangrove plant of the sundarbans tauhidur rahman nurunnabi 1, shaymaa almajmaie 2, ismini nakouti 2, lutfun nahar 2, s. The stem bark of this plant shows antimicrobial activity, which was also confirmed by preliminary data from our group results not published. Biodiversity and antimicrobial activity of endophytic fungi in angelica sinensis jiang shu, qian dawei, yang nianyun, tao jinhua, duan jinao jiangsu key laboratory for high technology research of traditional chinese medicine formulae, nanjing university of chinese medicine, nanjing 210023, china. In vitro antioxidant and antibacterial activity of. Among 65 endophytic fungi isolated, 18 fungi showed activity against at least one tested microorganism in preliminary screening, and the best results were obtained with nigrospora sphaerica urm6060 and. The antimicrobial activity of these endophytic microorganisms could be exploited in biotechnology, medicine, and agriculture. Isolation and antibacterial activity of endophytic fungi from. Isolation of endophytic fungi from xylocarpus granatum and.
Endophytic fungi reside in the intercellular space of plant nourished by the plant. Ophiopogon japonicus, containing compounds with antibacterial activity, is a. Antibacterial activity of endophytic fungi isolated from. The aim of this study was to screen the extracts of conifer.
Antimicrobial and phytochemical 1 screening of endophytic. Among seventeen endophytic fungi, only four distinct fungal morphotype were cultured to examine their antimicrobial properties and phytochemical analysis. Antimicrobial activity of endophytic fungi isolated and. Evaluation of antimicrobial activity of endophytic fungi. Antimicrobial activity of endophytic fungi from coffee plants article pdf available in bioscience journal 332. For example, fusarium solani ynas09 displayed antimicrobial activity to b. Pdf fungal endophytes of medicinal plants are important sources of secondary metabolites of pharmaceutical importance.
Antimicrobial activity of endophytic fungi associated with. Diversity and characterization of endophytic fungi. Gao 1school of plant protection, anhui agricultural university, hefei, china 2school of life science, anhui agricultural university, hefei, china. A total seventeen endophytic fungi isolated from spikes of the pinus roxburghii from pauri, garhwal region of uttrakhand. Therefore, it could be suggested that the antibacterial activities exhibited in mangrove leaf extracts were probably contributed by the endophytic fungi living in them. Research article open access antimicrobial activities of. Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential saringan awal kulat bakau endofit untuk potensi sebatian antimikrob dan bioserapan logam berat onn, m. Our research supports the previous finding that endophytic fungi display high antimicrobial activities. Diversity estimation and antimicrobial activity of culturable. Antimicrobial activity of cultivable endophytic fungi. The secondary metabolites of endophytic fungi from o. In total, 382 filamentous fungi and yeast isolates were obtained and cultured to examine the production of crude extracts.
Of the seven endophytic fungi isolated, four were found to exhibit anti bacterial activity. A total of 60 endophytic fungi produced secondary metabolites. Antimicrobial activity antimicrobial activity of crude extracts was evaluated by means of agarwell diffusion assay basha et al. Among these three isolates also showed anti oxidant activity. At various concentrations, the crude extract of fungi was tested against staphylococcus aureus, bacillus subtilis, streptococcus faecalis gram. It is a well known plant which possesses diverse health and industrial benefits. Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk medicine to treat various diseases. Antimicrobial activity of crude extracts of endophytic. It also confirmed that the antimicrobial activity is attributable to the presence of endophytic fungi in the plant. Endophytic fungi from the root tubers of medicinal plant. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Antimicrobial activity of saponins produced by two novel. All endophytes exhibited antibacterial activity on at least one of the test patho. Isolated from medicinal honeysuckle plant huiru zhang1, xinchen sun2 and chunping xu2, 1 college of bioengineering, henan university of technology, zhengzhou, henan, 450001, p.
Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk. In our previous reports, we have been studied the antimicrobial activity of the endophytic fungi isolated from the stems and leaves of s. The remaining fungal extracts exhibited significant level of total phenolic and flavonoid content, antioxidant and antibacterial activity. Abstractendophytes in plants may be coproducer of the bioactive compounds of their hosts. Antimicrobial activities of endophytic fungi obtained from the arid zone invasive plant opuntia dillenii and. Initial screening of mangrove endophytic fungi for antimicrobial. Abstract endophytes are microorganisms which inhabit inside plants. Biological activity and production of metabolites from. The results clearly showed that the endophytic fungi have stronger inhibition against plant pathogenic fungi, compared to human pathogenic bacteria. According to microbial taxonomy and morphology method, 10 species rhizoctonia dc of deuteromycotina, penicillium 6 species, 1 species of aspergillus, 1 species trichoderma and 2 species mucor of zygomycotina were identified with microscopic examination.
Antimicrobial activity was used as pointer to compare between endophytic activities of different strains in this study. A total of 56 isolates were obtained and 12 different. Biological activities from extracts of endophytic fungi. Cassia fistula is well known for its wealth of antimicrobial. Antimicrobial activities of endophytic fungi of the sri. Pdf antimicrobial activity of endophytic fungi from coffee. The outcomes of our study indicate that endophytic fungi have. Antimicrobial activity test of endophytic fungi strains the method of su et al. Isolation and identification of endophytic fungi from medicinal plants and assessment of their antimicrobial activity. Antibacterial activity of endophytic fungi isolated from the bark of. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. Yx28 and identification of its antimicrobial compound 7amino4.
Four of them showed an activity in the antagonist test against escherichia coli, pseudomonas aeruginosa, vibrio cholerae and shigelladysentriae. Antimicrobial activity of crude extracts of endophytic fungi from s. In return, they provide bioactive molecules which can play critical roles on plant defense system. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. The highest antimicrobial activity was shown against e. Antimicrobial activity of an endophytic xylaria sp. Antibacterial activity of endophytic fungi from leaves of. Antimicrobial activity in cultures of endophytic fungi. Antimicrobial activity was used as pointer to compare between endophytic activities of different strains in.
Diversity and antimicrobial activity of endophytic fungi. Ophiopogon japonicus, endophytic fungi, peptide deformylase, antimicrobial activity background endophytes are microorganisms that live in the intercellular spaces of healthy host tissues without causing obvious symptoms 1. Antimicrobial and antitumor activity and diversity of. Endophytic fungithe treasure chest of antibacterial substances. The purpose of the present study was to extract, explore and characterize antimicrobial activity produced by the endophytic fungi isolated from salt marsh plant leaves and stems of suaeda maritima and suaeda monoica. Pdf antimicrobial activity of metabolites of an endophytic. Antimicrobial and cytotoxic activities of endophytic. The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of orchidaceae collected in a brazilian tropical ecosystem.
Secondary metabolites produced by endophytic fungi have a broad spectrum of bioactivity, such as for antimicrobial, antioxidant, antitumor, enzymatic and surfactant compounds canuto. Antimicrobial activity of endophytic fungi from ferula foetida l. No reports are available on antimicrobial activities of the endophytic fungi isolated from e. Nevertheless, endophytic microorganisms can be pathogens, commensals, or mutualists, depending on plantendophyte interactions. Antimicrobial activities of endophytic fungi isolated from. The fungal culture was extracted with ethyl acetate and used as a crude extract for checking antimicrobial activities by well diffusion method. The aim of the present study was to screen for antimicrobial activity in endophytic fungi isolated from surface sterilized leaves and branches of five garcinia plants, g. Fifty six endophytes were isolated from the leaves, root, bark and fruits of sonneratia apetala, a pioneer mangrove plant in the sundarbans, bangladesh. Briefly, under aseptic conditions, 2 ml of microbial suspension indicator microbes, 1 x 106 cfuml was added to 100 ml culture media at approximately 50c, mixed rapidly, and then loaded onto. Antimicrobial activity of endophytic fungi from ferula foetida. Based on antimicrobial activity that showed in table 1, six extracts of fungi were active against e. Antimicrobial activity of metabolites of an endophytic fungus isolated from the leaves of citrus jambhiri rutaceae article pdf available march 2018 with 552 reads how we measure reads. Pdf antimicrobial activity of endophytic fungi from.
This study aims to identify endophytic fungi from medicinal plant mellochiaumbellataand investigate their antibacterial potencies. Antimicrobial activity of endophytic fungi isolated. This claim was supported by the findings of a group of chinese researchers who isolated endophytic fungi from several chinese medicinal plants, and further tested the. Cladpsporium cladosporioides had shown antimicrobial activity against pseudomonas aeruginosa and showed. Antimicrobial activity of endophytic fungi isolated from. About 17 80 95% endophytic fungi showed activity against one or more bacteria, and 14 66 67% endophytic fungi exhibited activity against one or more fungal pathogens. Antimicrobial activity of the ethanol extract of the fungal fermentation broth at a concentration of 10011gdisk was tested with the agar diffusion method against s. Biodiversity and antimicrobial activity of endophytes. The antimicrobial activity of 21 endophytic fungi was tested and shown in table 4. Furthermore, antimicrobial metabolites produced by the fermentation of endophytic fungi have many advantages, including no destruction of resources, sustainable use, easy largescale industrial production and quality control. Further, it is confirmed that the antimicrobial activity is attributable to the presence of endophytic fungi. Endophytic fungi are a promising source of new bioactive metabolites siqueira et al.
Highly potential fungus which showed antibacterial activities at the lowest amount. Antibacterial and antioxidant potential of endophytic. Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Pdf fungi, in particular endophytes are a promising source of new antimicrobial compounds. Antimicrobial activity of endophytic fungi isolated from the. Molecular characterization and analysis of antimicrobial. Antimicrobial activity of kojic acid from endophytic. Antimicrobial activity of crude extracts of endophytic fungi isolated. Antimicrobial activity of secondary metabolites of fungi isolated from leaves of bush mango chioma nwakanma 1, njoku en2 and pharamat t 3 1department of environmental management and toxicology, michael okpara university of agriculture, umudike, abia state, nigeria 2department of biological sciences, godfrey okoye university, enugu state, nigeria 3faculty of science, chulalongkorn university. Various medicinal plant species are moving from fringe to mainstream and being used by a large numbers of people who are seeking remedies and health approaches from natural products. The purpose of the present study was to extract, explore and characterize antimicrobial activity produced by the endophytic fungi isolated from salt marsh plant leaves.
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