For example, fusarium solani ynas09 displayed antimicrobial activity to b. A total seventeen endophytic fungi isolated from spikes of the pinus roxburghii from pauri, garhwal region of uttrakhand. Endophytic fungi that showed antimicrobial activity were identified based on analysis of the dna sequences of the its15. At various concentrations, the crude extract of fungi was tested against staphylococcus aureus, bacillus subtilis, streptococcus faecalis gram. A total of 60 endophytic fungi produced secondary metabolites. Isolation and antibacterial activity of endophytic fungi from. Nevertheless, endophytic microorganisms can be pathogens, commensals, or mutualists, depending on plantendophyte interactions. Antimicrobial activity of endophytic fungi associated with. Method endophytic fungi were isolated from the stem, leaves and root of angelica sinensis by the surface sterilization method and scoring method. Yx28 and identification of its antimicrobial compound 7amino4. Antimicrobial activity of kojic acid from endophytic. Antimicrobial activity of kojic acid from endophytic fungus colletotrichum gloeosporioides isolated from sonneratia apetala, a mangrove plant of the sundarbans tauhidur rahman nurunnabi 1, shaymaa almajmaie 2, ismini nakouti 2, lutfun nahar 2, s.
The stem bark of this plant shows antimicrobial activity, which was also confirmed by preliminary data from our group results not published. Evaluation of antimicrobial activity of endophytic fungi. Hence, in this paper we describe the isolation and identification of the endophytic fungi of the aquatic plant n. Biodiversity and antimicrobial activity of endophytes. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a. Molecular characterization and analysis of antimicrobial. Antimicrobial and phytochemical 1 screening of endophytic. Antimicrobial activity of an endophytic xylaria sp.
Isolated from medicinal honeysuckle plant huiru zhang1, xinchen sun2 and chunping xu2, 1 college of bioengineering, henan university of technology, zhengzhou, henan, 450001, p. The aim of this study was to screen the extracts of conifer. Antimicrobial activity in cultures of endophytic fungi isolated from. Antimicrobial activities of endophytic fungi obtained from the arid zone invasive plant opuntia dillenii and. Antimicrobial activity of endophytic fungi isolated. Among seventeen endophytic fungi, only four distinct fungal morphotype were cultured to examine their antimicrobial properties and phytochemical analysis.
Pdf antimicrobial activity of endophytic fungi from coffee. The rafflesia flower is the largest single flower in the world, a parasitic flowering plant found only in borneo, indonesia, malaysia and philippines. The seven endophytic fungi were subjected for antimicrobial activity against pathogenic bacteria viz. Therefore, it could be suggested that the antibacterial activities exhibited in mangrove leaf extracts were probably contributed by the endophytic fungi living in them. In total, 382 filamentous fungi and yeast isolates were obtained and cultured to examine the production of crude extracts. Diversity and characterization of endophytic fungi.
Antimicrobial assay thirty nine isolates of endophytic fungi were randomly chosen to estimate their ability to produce antimicrobial compounds antibacterial or antifungal activity. Antibacterial activity of endophytic fungi isolated from the bark of. Pdf antimicrobial activity of endophytic fungi isolated from. Antimicrobial activity of endophytic fungi from coffee plants article pdf available in bioscience journal 332. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. The secondary metabolites of endophytic fungi from o. Furthermore, antimicrobial metabolites produced by the fermentation of endophytic fungi have many advantages, including no destruction of resources, sustainable use, easy largescale industrial production and quality control. Antimicrobial activity of crude extracts of endophytic fungi. Antimicrobial activity of cultivable endophytic fungi. Mycelial extracts mc and fermentation broth fb were tested for antimicrobial activity using peptide deformylase pdf inhibition fluorescence. The current study determined the antimicrobial properties and identify the chemical compounds of secondary metabolites produced by endophytic fungi isolated from sceletium tortuosum l. Fifty six endophytes were isolated from the leaves, root, bark and fruits of sonneratia apetala, a pioneer mangrove plant in the sundarbans, bangladesh. The remaining fungal extracts exhibited significant level of total phenolic and flavonoid content, antioxidant and antibacterial activity. Endophytic fungi reside in the intercellular space of plant nourished by the plant.
The bioactive abilities of endophytic fungi were not identical, even though they were isolated from the same host species, and belonged to the same genus. Pdf antimicrobial activity of metabolites of an endophytic. Antibacterial activity of endophytic fungi isolated from. Antimicrobial activities of endophytic fungi of the sri. A total of 56 isolates were obtained and 12 different. Antimicrobial activity of endophytic fungal islate in argemone maxicana. Endophytic fungithe treasure chest of antibacterial substances. Endophytic fungi have the ability to coexist with their host plants without causing any harm and are beneficial to both the plant and the fungi. The antimicrobial activity of these endophytic microorganisms could be exploited in biotechnology, medicine, and agriculture. Further, it is confirmed that the antimicrobial activity is attributable to the presence of endophytic fungi. Endophytic fungi are a promising source of new bioactive metabolites siqueira et al. Pdf antimicrobial activity of endophytic fungi from.
The antimicrobial activity of 21 endophytic fungi was tested and shown in table 4. According to microbial taxonomy and morphology method, 10 species rhizoctonia dc of deuteromycotina, penicillium 6 species, 1 species of aspergillus, 1 species trichoderma and 2 species mucor of zygomycotina were identified with microscopic examination. Detection of secondary metabolites content of each endophytic fungi species. The highest antimicrobial activity was shown against e.
Briefly, under aseptic conditions, 2 ml of microbial suspension indicator microbes, 1 x 106 cfuml was added to 100 ml culture media at approximately 50c, mixed rapidly, and then loaded onto. Endophytic fungi from the root tubers of medicinal plant. Antimicrobial activity of endophytic fungi isolated and. These microorganisms can be useful for prospectionof bioactive. Antimicrobial activity of saponins produced by two novel. Evaluation of antimicrobial activity of endophytic fungi from. Cladpsporium cladosporioides had shown antimicrobial activity against pseudomonas aeruginosa and showed. The present study is aimed at the isolation and identification of endophytic fungi from p. It is a well known plant which possesses diverse health and industrial benefits. Antimicrobial activity of metabolites of an endophytic fungus isolated from the leaves of citrus jambhiri rutaceae article pdf available march 2018 with 552 reads how we measure reads. About 17 8095% endophytic fungi showed activity against one or more bacteria, and 14 6667% endophytic fungi exhibited activity against one or more fungal pathogens.
Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional. Among these three isolates also showed anti oxidant activity. The agar plate diffusion assay was used to evaluate the antimicrobial activity rios et al. The purpose of this study was to examine antimicrobial activity of endophytic fungi isolated from the leaves, stems, and roots of 54 species of orchidaceae collected in a brazilian tropical ecosystem.
Antimicrobial activity was used as pointer to compare between endophytic activities of different strains in. Initial screening of mangrove endophytic fungi for antimicrobial. Gao 1school of plant protection, anhui agricultural university, hefei, china 2school of life science, anhui agricultural university, hefei, china. The purpose of the present study was to extract, explore and characterize antimicrobial activity produced by the endophytic fungi isolated from salt marsh plant leaves and stems of suaeda maritima and suaeda monoica. Ophiopogon japonicus, endophytic fungi, peptide deformylase, antimicrobial activity background endophytes are microorganisms that live in the intercellular spaces of healthy host tissues without causing obvious symptoms 1. Isolation of endophytic fungi from xylocarpus granatum and. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. Of the seven endophytic fungi isolated, four were found to exhibit anti bacterial activity. Research article open access antimicrobial activities of. Pn17, were isolated from traditional chinese medicinal herb p.
The results clearly showed that the endophytic fungi have stronger inhibition against plant pathogenic fungi, compared to human pathogenic bacteria. Antibacterial and antioxidant potential of endophytic fungi. This could be because the endophytic fungi and plant pathogenic fungi naturally exist in the same habitat. Antimicrobial activity in cultures of endophytic fungi. Antimicrobial activity of endophytic fungi from ferula foetida l. In our previous reports, we have been studied the antimicrobial activity of the endophytic fungi isolated from the stems and leaves of s. Various medicinal plant species are moving from fringe to mainstream and being used by a large numbers of people who are seeking remedies and health approaches from natural products. Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk. C for 36 days and were inoculated into ml flasks containing 200gms of unpolished rice, soaked with 200 ml distilled water autoclaved. Endophytic fungi isolation from the meliaceae plant xylocarpus granatum was investigated and 20 potential pure strains were isolated. Antimicrobial activity of endophytic fungi the antimicrobial activity of 21 endophytic fungi was tested and shown in table 4. All endophytes exhibited antibacterial activity on at least one of the test patho.
The present study helped to justify the traditional use of vitex negundo l. We conducted a study to bioprospect for saponinproducing endophytic fungi from panax notoginseng and evaluate the antimicrobial activity of saponins. Molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia. Antimicrobial activity of crude extracts of endophytic fungi isolated. Biodiversity and antimicrobial activity of endophytic fungi. Antimicrobial activity of crude extracts of endophytic fungi from s. Antimicrobial activity of endophytic fungi from ferula foetida. Antimicrobial activity was used as pointer to compare between endophytic activities of different strains in this study. The etoac extracts of all endophytic fungi and isolated compounds were screened for antimicrobial activity against s.
Among 65 endophytic fungi isolated, 18 fungi showed activity against at least one tested microorganism in preliminary screening, and the best results were obtained with nigrospora sphaerica urm6060 and. Antimicrobial and enzyme activity of endophytic fungi isolated from tulsi. Antimicrobial activity antimicrobial activity of crude extracts was evaluated by means of agarwell diffusion assay basha et al. Antimicrobial activity test of endophytic fungi strains the method of su et al. Antibacterial and antioxidant potential of endophytic. This study shows that the endophytic fungi isolated. Cassia fistula is well known for its wealth of antimicrobial. Four of them showed an activity in the antagonist test against escherichia coli, pseudomonas aeruginosa, vibrio cholerae and shigelladysentriae. Isolation and identification of endophytic fungi from medicinal plants and assessment of their antimicrobial activity.
Antitumor and antimicrobial activities of endophytic fungi. Antimicrobial activity of secondary metabolites of fungi isolated from leaves of bush mango chioma nwakanma 1, njoku en2 and pharamat t 3 1department of environmental management and toxicology, michael okpara university of agriculture, umudike, abia state, nigeria 2department of biological sciences, godfrey okoye university, enugu state, nigeria 3faculty of science, chulalongkorn university. Antimicrobial activity of endophytic fungi isolated from the. Antimicrobial activities of endophytic fungi isolated from. Pdf isolation and identification of endophytic fungi. Based on antimicrobial activity that showed in table 1, six extracts of fungi were active against e.
Antimicrobial and cytotoxic activities of endophytic. Antimicrobial activity of the ethanol extract of the fungal fermentation broth at a concentration of 10011gdisk was tested with the agar diffusion method against s. Isolation and antibacterial activity of endophytic fungi. Secondary metabolites produced by endophytic fungi have a broad spectrum of bioactivity, such as for antimicrobial, antioxidant, antitumor, enzymatic and surfactant compounds canuto.
Initial screening of mangrove endophytic fungi for antimicrobial compounds and heavy metal biosorption potential saringan awal kulat bakau endofit untuk potensi sebatian antimikrob dan bioserapan logam berat onn, m. The fungal culture was extracted with ethyl acetate and used as a crude extract for checking antimicrobial activities by well diffusion method. Biological activities from extracts of endophytic fungi. Study on angelica sinensis endophytic fungi and its. Occurrence, classification, function and natural products. The fresh mycelia of endophytic fungi were grown on pda plates at 282. Pdf fungi, in particular endophytes are a promising source of new antimicrobial compounds. Diversity and antimicrobial activity of endophytic fungi. The aim of the present study was to screen for antimicrobial activity in endophytic fungi isolated from surface sterilized leaves and branches of five garcinia plants, g. Antimicrobial activity of secondary metabolites of fungi isolated from leaves of bush mango chioma nwakanma 1, njoku en2 and pharamat t 3 1department of environmental management and toxicology, michael okpara university of agriculture, umudike, abia state, nigeria. Twelve species of endophytic fungi weresuccessfully isolated from mellochiaumbellataleaves and twigs. Antimicrobial activity of crude extracts of endophytic.
Pdf fungal endophytes of medicinal plants are important sources of secondary metabolites of pharmaceutical importance. It also confirmed that the antimicrobial activity is attributable to the presence of endophytic fungi in the plant. In return, they provide bioactive molecules which can play critical roles on plant defense system. Biodiversity and antimicrobial activity of endophytic.
Pdf antibacterial activity of endophytic fungi isolated from conifer. Diversity estimation and antimicrobial activity of culturable. Antimicrobial and antitumor activity and diversity of. Our research supports the previous finding that endophytic fungi display high antimicrobial activities. Gulyamova1 1department of biochemistry and biotechnology of physiologically active compounds, institute of microbiology of the academy of sciences ru, uzbekistan. Endophytic microorganisms are fungi or bacteria which live inside plant tissues or organs, without causing them any harmful symptoms. Initial screening of mangrove endophytic fungi for. This study aims to identify endophytic fungi from medicinal plant mellochiaumbellataand investigate their antibacterial potencies.
Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk medicine to treat various diseases. Antimicrobial activity and phytochemical analysis of crude. In this study, we evaluated the antimicrobial activity of endophytic fungi isolated from h. In total, 382 filamentous fungi and yeast isolates were obtained and cultured to. Each endophytic fungi isolates were inoculated in potato dextrose broth pdb. Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant trichilia elegans a.
Antimicrobial activity screening of endophytic fungi extracts. Endophytic fungi exhibiting broad spectrum of antimicrobial activity were subjected for the production of secondary metabolites. The study helped to justify the traditional use of ocimum sanctum l. Antimicrobial activities of endophytic fungi isolated from ophiopogon. Biodiversity of endophytic fungi in different leaf ages of. Natural products from entophytic microbes have been observed to inhibit or kill wide variety of harmful disease. About 17 80 95% endophytic fungi showed activity against one or more bacteria, and 14 66 67% endophytic fungi exhibited activity against one or more fungal pathogens. Highly potential fungus which showed antibacterial activities at the lowest amount. Biodiversity and antimicrobial activity of endophytic fungi in angelica sinensis jiang shu, qian dawei, yang nianyun, tao jinhua, duan jinao jiangsu key laboratory for high technology research of traditional chinese medicine formulae, nanjing university of chinese medicine, nanjing 210023, china.
The outcomes of our study indicate that endophytic fungi have. Abstract endophytes are microorganisms which inhabit inside plants. Antimicrobial activity of endophytic fungi isolated from. Biological activity and production of metabolites from. Pdf isolation and identification of endophytic fungi from. Jan 02, 2016 endophytic fungi were tested for antifungal activity using a dual culture method. Abstractendophytes in plants may be coproducer of the bioactive compounds of their hosts. In vitro antioxidant and antibacterial activity of. Antibacterial activity of endophytic fungi from leaves of.
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